Journal: Materials Today Bio

Article Title: Stepwise targeting hyaluronic acid-based polymeric prodrug for acute kidney injury and prevents its progression to chronic kidney disease

doi: 10.1016/j.mtbio.2025.102207

Figure Lengend Snippet: THTM ameliorates renal fibrosis in AKI to CKD. (A) A schematic summary of the administration schedule used for THTM against CKD. (B) Serum creatinine (CRE) and (C) blood urea nitrogen (BUN) in CKD mice. (D) SOD activity and (E) MDA levels in the renal tissues of Cis-AKI mice. (F) Masson staining of renal tissues of CKD mice. (G) Immunofluorescence staining (green) with α-SMA, indicative of suppressed myofibroblast activation. (H) The changes of TGF-β1 in renal tissues of CKD mice detected by ELISA. Scale bar 100 μm. Data are presented as means ± SD, n = 6. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: Anti-CD44 (15675-1-AP, 30854-1-AP), anti-KIM-1 (30948-1-AP) and anti-α-SMA (14395-1-AP) antibodies were purchased from Proteintech (China).

Techniques: Activity Assay, Staining, Immunofluorescence, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: Regenerative Therapy

Article Title: In vitro effects of conditioned medium from bioreactor cultured human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on skin-derived cell lines

doi: 10.1016/j.reth.2021.08.003

Figure Lengend Snippet: Characterization of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) at Passage 2. A . The hUC-MSCs were isolated and cultured from the umbilical cord tissue. Morphology of hUC-MSCs shows successful proliferation. (Scale bar: 300 μm, 100 μm, 50 μm). B. Immunohistochemistry staining of hUC-MSCs with antibodies providing positive or negative signals. MSCs were subjected to immunolocalization of MSCs specific surface antigens using primary antibodies directed against CD 105, CD34, CD45, and CD44 and stained by secondary antibodies conjugated with FITC/Rhodamine and counterstained with DAPI. Positive markers CD105 and CD44 were expressed, and negative markers CD34 and CD45 were not expressed. (Scale bar: 50 μm). C. hUC-MSCs characterization by fluorescence-activated cell sorting (FACS). Expression level of surface markers is maintained during in vitro culture of hUC-MSCs. Positive markers CD44 and CD105 were expressed as a high percentage, and negative markers CD45 and CD34 were detected as a low percentage.

Article Snippet: For fluorescence-activated cell sorting (FACS), the sheep anti-human α-CD44 antibody conjugated with PE (R&D, Minneapolis, Minnesota, USA), rat anti-human α-CD34 antibody conjugated with PE (R&D, Minneapolis, Minnesota, USA), rat anti-human α-CD105 antibody conjugated with FITC (Abcam, Cambridge, MA, USA), and rat anti-human α-CD45 antibody conjugated with PE (R&D, Minneapolis, Minnesota, USA) were used.

Techniques: Derivative Assay, Isolation, Cell Culture, Immunohistochemistry, Staining, Fluorescence, FACS, Expressing, In Vitro